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General description
Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques such as Golgi staining and modern molecular approaches such as GFP constructs yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, Millipore has developed a polyclonal antibody blend that reacts against key somatic, nuclear, dendritic, and axonal proteins distributed across the pan-neuronal architecture that can then be detected by a single secondary antibody. This antibody cocktail has been validated in diverse methods, cell culture and immuno-histochemistry, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology.
Immunogen
Epitope: Whole Neuron Marker
Application
Neuro-Chrom Pan Neuronal Marker-Rabbit, Alexa488 conjugate is an antibody targeting the Pan Neuronal Marker protein, validated for use in ICC, IHC & IF.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Biochem/physiol Actions
Cat. # ABN2300A4 is specific to axons (neurites), dendrites, nucleus, and the cell body of neurons.
Reactivity with other species has not been determined.
Physical form
Polyclonal antibody cocktail blend conjugated to Alexa Fluor™ 488 in PBS buffer with 15mg/mL BSA and 0.05% NaN3
Preparation Note
Maintain at 2-8°C for up to 1 year from date of receipt.
Analysis Note
Control
Rat E18 cortex primary neuron cell culture or mouse adult brain cryosections.
Routinely tested Rat E18 cortex primary neurons in Immunocytochemistry.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| clone | polyclonal |
| species reactivity | mouse, rat |
| manufacturer/tradename | Neuro-Chrom |
| technique(s) | immunocytochemistry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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