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General description
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
Immunogen
Human embryonal carcinoma cell line 2102Ep
Application
Detect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, Alexa Fluor 488 Conjugate validated for use in IC.
Research Category
Neuroscience
Biochem/physiol Actions
This antibody reacts with TRA-1-60 antigen expressed upon the surface of human EC, EG, and ES cells. No immunoreactivity is seen with murine EC, EG or ES cells. TRA-1-81 (MAB4381A4) and TRA-1-60 monoclonal antibodies recognize antigens that are associated with a pericellular matrix proteoglycan.
Physical form
Purified mouse monoclonal IgM conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
size exclusion
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
H9 human embryonic stem cells
Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TRA-1-60 in H9 human embryonic stem cells.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | TRA - 1-60, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| isotype | IgM |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PODXL(5420) |
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¥ 1259.40
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¥ 4039.86
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¥ 3275.78
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¥ 3372.68
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¥ 4897.21
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¥ 2842.54
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¥ 1509.00
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¥ 8137.12
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