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General description
The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFE3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta. Recently, Smith et al. have proven that the exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor TFE3 (Cell 153, 335–347, April 11, 2013).
~ 65 kDa observed
Immunogen
linear peptide corresponding to Human TFE3.
Application
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory on TFE3 overexpressing cell lines, epiblast and primitive endodermal cells (Smith, A., et al. (2013). Cell. 153, 335–347).
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-TFE3 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of TFE3.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS, 50% glycerol, and 0.05% sodium azide
Preparation Note
Store at -20°C
Analysis Note
Evaluated by Western Blot in a HEK293T cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected TFE3 in 10 µg of HEK293T cell lysate.
Uncharacterized band(s) may appear in some lysates with longer exposure times.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_006512 |
| UniProt accession no. | P19532 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TFE3(7030) |
| 长度(mm) | |
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