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General description
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE341.
Refer to Cat. No. MABE341 for observed molecular weight information.
Immunogen
Puromycin-incorporated neosynthesized proteins at multiple molecular weights
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.
Biochem/physiol Actions
Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
This antibody detects Puromycin-incorporated neosynthesized proteins.
Physical form
Protein G Purified
Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.
Alexa Fluor™ is a registered trademark of Life Technologies
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 17H1, monoclonal |
| species reactivity | human |
| concentration | 0.5 mg/mL |
| technique(s) | immunocytochemistry: suitable |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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