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General description
Members of the F-box protein family, such as SCRAPPER, are characterized by an approximately 40-amino acid F-box motif. SCRAPPER is selectively expressed in the brain, broadly expressed within the mouse CNS, and is abundant at the presynaptic membrane. It has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. SCRAPPER is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
~48 kDa observed. Uncharacterized bands may appear at ~52 kDa, ~58 kDa and ~70 kDa in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human SCRAPPER.
Application
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This SCRAPPER antibody is validated for use in WB & IHC for the detection of the SCRAPPER protein.
Western Blot Analysis: 1 µg/mL from a representative lot detected SCRAPPER in 10 µg/mL of RAW264.7 cell lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected SCRAPPER in human midbrain tissue.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human spleen tissue lysate
Evaluated by Western Blot in human spleen tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected SCRAPPER in 10 µg/mL of human spleen tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| UniProt accession no. | Q96IG2 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FBXL20(84961) |
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