- 商品介绍
- 规格参数
- 包装参数
Immunogen
FM9 melanoma cells
Application
Anti-Disialoganglioside GD3 Antibody, clone MB3.6 is an antibody against Disialoganglioside GD3 for use in FC, IF, IH.
Immunohistochemistry (frozen tissue sections)
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.
Physical form
Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | MB3.6, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable |
| isotype | IgG3 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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| 重量(kg) |
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