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- 规格参数
- 包装参数
General description
Cullin-4A (UniProt Q13619; also known as CUL-4A) is encoded by the CUL4A gene (Gene ID 8451) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type (really interesting new gene), HECT-type (homologous to E6-AP C-terminus), and U-box type of E3 ligases. Cullin-4 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The human genome encodes 8 cullin proteins (CUL 1, 2, 3, 4A, 4B, 5, 7 and 9). The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). CUL4A interacts with the substrate-binding adaptor DDB1 (DNA damage binding protein 1) via its arc-shaped helical N-terminal domain, and binds the small RING finger protein ROC1 (regulator of cullins 1) via its globular C-terminal domain. Despite the 83% sequence identity and seemingly functional redundancy between CUL4A and CUL4B, much more focus is placed on CUL4A in oncogenesis research.
~88 kDa observed
Immunogen
Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human Cullin-4A/CUL4A near the C-terminus.
Application
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Cullin-4A/CUL4A Antibody, clone 2A2.1 is validated for use in Western Blotting for the detection of Cullin-4A/CUL4A.
Biochem/physiol Actions
Clone 2A2.1 is expected to react with both spliced isoforms of human cullin-4A
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human melanoma SK-MEL-28 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cullin-4A/CUL4A in 10 µg of human melanoma SK-MEL-28 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 2A2.1, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001008895 |
| UniProt accession no. | Q13619 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CUL4A(8451) |
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