- 商品介绍
- 规格参数
- 包装参数
Immunogen
Epitope: a.a. 151-165
Application
Immunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000
Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000
ELISA at 1:1,000-1:2,000
Western blot analysis at 1:1,000-1:2,000
Immunoprecipitation at 1:1-1:10
Gel super shifts at 1:1-1:10
Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER.
Biochem/physiol Actions
The peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors.
SPECIES REACTIVITIES:
Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.
Physical form
Ascites. Liquid, does not contain any preservative.
Preparation Note
Maintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | B10, monoclonal |
| species reactivity | rat, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2a |
| NCBI accession no. | NP_000116.2 |
| UniProt accession no. | P03372 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HSPG2(3339) |
| 长度(mm) | |
| 宽度(mm) | |
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