- 商品介绍
- 规格参数
- 包装参数
General description
Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
Immunogen
Chymase purified from human skin.
Application
Immunohistochemistry at 1:2,000:1:4,000.
For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.
Biochem/physiol Actions
Reacts with human mast cell chymase.
Physical form
Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Analysis Note
Control
POSITIVE CONTROL: lung, heart, skin, placenta.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | biotin conjugate |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | B7, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | dot blot: suitable,immunohistochemistry: suitable |
| isotype | IgG1κ |
| suitability | not suitable for Western blot |
| NCBI accession no. | NM_001836.2 |
| UniProt accession no. | P23946 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CMA1(1215) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
询价
-
询价
-
¥ 2013.69
-
¥ 5887.85
-
询价
-
询价
-
¥ 238.17
-
¥ 2883.61
-
¥ 3812.28
-
询价
-
¥ 2617.87
-
¥ 3476.87
-
¥ 954.17
-
¥ 5804.96
-
询价
-
¥ 29754.56
-
询价
-
¥ 14722.42
-
¥ 57595.03
微信小程序
7X24小时在线咨询