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- 规格参数
- 包装参数
General description
PROPERTIES:
Binds to heparin. Active in cell attachment of MG63 cells.
Product Source: Human plasma; tested negative for antibodies to HBsAg and HIV.
Vitronectin (S-protein) is a multi-functional plasma and extracellular matrix protein with defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to other macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.
Application
Suitable for use in ELISA, immunization, cell attachment assays and electrophoresis.
Physical form
Liquid in 50 mM sodium phosphate, 100 mM NaCl, pH 7.4, containing no preservatives.
Preparation Note
Stable 12 months at -70°C. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Analysis Note
Two bands in SDS-PAGE at 65 kDa and 75 kDa.
Other Notes
Replaces: 08-126
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Level | 100 |
| assay | ≥90% (SDS-PAGE) |
| form | liquid |
| specific activity | 1 μg/mL (cell attachment assays) |
| manufacturer/tradename | Chemicon® |
| concentration | 1 mg/mL |
| technique(s) | cell culture | mammalian: suitable |
| input | sample type hematopoietic stem cell(s) sample type mesenchymal stem cell(s) sample type: human embryonic stem cell(s) sample type pancreatic stem cell(s) sample type epithelial cells sample type induced pluripotent stem cell(s) sample type neural stem cell(s) |
| NCBI accession no. | NM_000638.3 |
| UniProt accession no. | P04004 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... VTN(7448) |
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