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General description
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
~39 kDa
Immunogen
Recombinant human Oct-4 protein, a.a. 1-145.
Application
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1].
Western Blotting: Recommended working dilution is 1 - 2 µg/mL.
Flow Cytometry: Recommended working dilution is 2 µg/mL.
Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.
Biochem/physiol Actions
Reactivity to other species has not been determined.
This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.
Physical form
Format: Purified
Protein G sepharose column
Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3.
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB4305
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7F9.2, monoclonal |
| species reactivity | human, mouse |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,western blot: suitable |
| input | sample type: human embryonic stem cell(s) sample type induced pluripotent stem cell(s) sample type: mouse embryonic stem cell(s) |
| isotype | IgG1κ |
| NCBI accession no. | NP_976034 |
| UniProt accession no. | P20263 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... POU5F1(5460) mouse ... Pou5F1(18999) |
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