- 商品介绍
- 规格参数
- 包装参数
General description
The Gβ5 (GNB5 guanine nucleotide binding protein (G protein), beta 5) is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa. It has also been found that Gβ5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas G1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. This antibody is specific for a peptide sequence within the D1 blade which is not found in Gβ1-4.
This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa
Immunogen
Epitope: D1
Unique peptide sequence derived from the D1 blade of the Gβ5 protein.
Application
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Gβ5 Antibody is validated for use in WB for the detection of Gβ5.
Biochem/physiol Actions
Recognizes D1 area of Gβ5.
Physical form
Format: Purified
Purified
Purified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
L6 Cell Lysate, plasma membranes from rat cerebrum.
Routinely evaluated by Western Blot on L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected G protein Gbeta5 on 10 μg of L6 lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB4068
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, rat, human |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_006578.3 |
| UniProt accession no. | O14775 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... GNB5(10681) mouse ... Gnb5(14697) rat ... Gnb5(83579) |
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