- 商品介绍
- 规格参数
- 包装参数
General description
SMC® 人TNFα高灵敏度免疫分析试剂盒包含进行分析所需的所有试剂。该测定采用以标准孔板形式配置的标准免疫测定工作流程。
测定方案与现有的夹心ELISA方法相似,但有两个主要区别:
1)洗脱缓冲液破坏夹心结构,分离标记的检测抗体以进行定量。
2)Erenna
Application
Inflammatory Disease / Immune Response / Autoimmune Disease
Features and Benefits
- Ultra-Sensitive Detection: Utilizing SMC®technology, this assay detects TNFα at femtogram-level concentrations, enabling precise quantification in human plasma and serum—even in low-expression conditions.
- Familiar Workflow, Elevated Performance: Follows a standard sandwich immunoassay protocol with a unique elution step and digital readout, delivering high signal-to-noise ratios and low background interference.
- Expert Scientific Support: Backed by Field Application Scientists and technical support teams experienced in academic, pharma, CRO, and regulated lab environments.
- Automation-Ready Workflow: Compatible with liquid handling systems for hands-free operation, reducing variability and increasing throughput.
Packaging
96 well assay
Analysis Note
- Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
- Standard Curve Range: 50-50,000 fg/mL
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Linearity: Serum: 113% Plasma: 105%
- Recovery: Serum: 95% Plasma: 83%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Although TNFα was detectable in non-human primate, rat, feline, canine and equine samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
我们的产品仅用于研究目的,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用对人类或动物。
| description | Used for ultrasensitive quantification of Human TNFα in human serum and plasma |
| product line | SMC® |
| usage | kit sufficient for 96 wells |
| species reactivity | human |
| technique(s) | single molecule counting: suitable |
| input | plasma (K2 EDTA) serum |
| UniProt accession no. | P01375 |
| compatibility | for use with FemtoQuest™ and SMCxPRO® instruments |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| 长度(mm) | |
| 宽度(mm) | |
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