SMC® 人TNFα 高灵敏度试剂盒,1KIT,03-0163-00,密理博

销售价: ¥ 10319.04 / 件
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订货号 0BV6816
品牌型号 密理博 03-0163-00
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

SMC® 人TNFα高灵敏度免疫分析试剂盒包含进行分析所需的所有试剂。该测定采用以标准孔板形式配置的标准免疫测定工作流程。

测定方案与现有的夹心ELISA方法相似,但有两个主要区别:
1)洗脱缓冲液破坏夹心结构,分离标记的检测抗体以进行定量。
2)Erenna 和SMCxPRO® 免疫测定系统使用单分子计数(SMC®)技术对分析物进行检测。


Application

Inflammatory Disease / Immune Response / Autoimmune Disease


Features and Benefits

  • Ultra-Sensitive Detection: Utilizing SMC®technology, this assay detects TNFα at femtogram-level concentrations, enabling precise quantification in human plasma and serum—even in low-expression conditions.
  • Familiar Workflow, Elevated Performance: Follows a standard sandwich immunoassay protocol with a unique elution step and digital readout, delivering high signal-to-noise ratios and low background interference.
  • Expert Scientific Support: Backed by Field Application Scientists and technical support teams experienced in academic, pharma, CRO, and regulated lab environments.
  • Automation-Ready Workflow: Compatible with liquid handling systems for hands-free operation, reducing variability and increasing throughput.


Packaging

96 well assay


Analysis Note

  • Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 50-50,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 113% Plasma: 105%
  • Recovery: Serum: 95% Plasma: 83%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.


Other Notes

Although TNFα was detectable in non-human primate, rat, feline, canine and equine samples, the assay has not been verified for these species.


Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

我们的产品仅用于研究目的,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用对人类或动物。


技术参数 Specifications
descriptionUsed for ultrasensitive quantification of Human TNFα in human serum and plasma
product lineSMC®
usage kit sufficient for 96 wells
species reactivityhuman
technique(s)single molecule counting: suitable
input plasma (K2 EDTA)
serum
UniProt accession no.P01375
compatibilityfor use with FemtoQuest and SMCxPRO® instruments
shipped inwet ice
storage temp.2-8°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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