Benzonase ® Salt Tolerant Endonuclease，40KU，E5014-40KU，Sigma

General description

The best-in-class solution for nucleic acid removal at high salt concentration. The Benzonase^® Salt Tolerant endonuclease is an enzyme, different in amino acids sequence from other Benzonase^® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations. The protein is a monomer with a molecular weight of about 27 kDa . The protein has an isoelectric point (pI) at pH 9.68. It is functional between pH 6 and 10, and from 0 °C to above 37 °C. Mg2+ (1–2 mM) is required for enzyme activity.

Application

Benzonase^® Salt Tolerant endonuclease degrades both DNA and RNA, whether single stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, Benzonase^® Salt Tolerant endonuclease hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate terminated oligonucleotides, which are three to five bases in length.

Features and Benefits

• Active at higher salt concentration, up to1M NaCl
• Non-animal origin, recombinant from E.Coli, High Purity
• No post-translational modifications
• High batch to batch reproducibility
• Free of detectable protease activity
• Does not contain any antimicrobial preservatives or protein stabilizers except glycerol
• Compatible with Deviron^® detergents for cell lysis

Preparation Note

-10°C to -25°C

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
DEVIRON is a registered trademark of Merck KGaA, Darmstadt, Germany