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- 规格参数
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General description
Tripartite motif-containing protein 5 (TRIM5), also known as RING finger protein 88 (RNF88), is a member of the TRIM/RBCC family defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. TRIM5 isoform alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection.
~55 kDa observed. Uniprot describes 6 isoforms ranging between ~30 kDa and ~56 kDa Uncharacterized bands may appear in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to the intermediate domain of human TRIM5 alpha.
Application
Detect Tripartite motif-containing protein 5 using this rabbit polyclonal antibody, Anti-TRIM5 alpha Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2 µg/mL from a representative lot detected TRIM5 alpha in human stomach tissue.
Immunofluorescence Analysis: 10 µg/mL from a representative lot detected TRIM5 alpha in human stomach cells.
Biochem/physiol Actions
This antibody recognizes the intermediate domain of TRIM5 alpha.
Analysis Note
Control
Human stomach, thymus, and uterus tissue lysates
Evaluated by Western Blotting in human stomach, thymus, and uterus tissue lysates.
Western Blotting Analysis: 2 µg/mL of this antibody detected TRIM5 alpha in 15 µg of human stomach, thymus, and uterus tissue lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| UniProt accession no. | Q9C035 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TRIM5(85363) |
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