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General description
G-protein coupled receptor kinase 2 (Grk2), also known as BARK or BARK1, is a member of the large family of GRK kinases that phosphorylate G-protein coupled receptors (GPCR), resulting in the recruitment of arrestins to the cytoplasmic domains of GPCR’s and subsequent internalization of these receptors by endocytosis. GRK2 is ubiquitously expressed, and modulates diverse cellular processes through association with a number of non-receptor substrates. GRK2 regulates actin dynamics and cell motililty through interaction with radixin, tubilin, and ezrin. In addition, Grk2 may play a role in the signaling of TGFβ by blocking the nuclear translocation of Smad2 and Smad3 proteins. It has also been shown that Grk2 may play a role in the hedgehog signaling pathway during embryonic development, by modulating the signaling of the atypical G-protein coupled receptor Smo. Grk2 is inactivated by phosphorylation of ser670 by ERK. It is also phosphorylated by PKC and Src, depending on the cellular context.
~70 kDa observed
Immunogen
Epitope: Phosphorylated Ser670
KLH-conjugated linear peptide corresponding to human GRK2 phosphorylated at Ser670.
Application
Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay.
Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot blocked phospho-GRK2 in 10 µg of Calyculin A/Okadaic Acid treated and untreated Jurkat cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-GRK2 in HeLa cell lysate.
Research Category
Signaling
Research Sub Category
Hormones & Receptors
Biochem/physiol Actions
This antibody recognizes GRK2 phosphorylated at Ser670.
Physical form
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Untreated and Calyculin A/Okadaic Acid treated A431 cells
Evaluated by Western Blot in untreated and Calyculin A/Okadaic Acid treated A431 cells.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected GRK2 in 10 µg of untreated and Calyculin A/Okadaic Acid treated A431 cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 15G11.1, monoclonal |
| species reactivity | human |
| technique(s) | immunoprecipitation (IP): suitable,inhibition assay: suitable (peptide),western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_001610 |
| UniProt accession no. | P25098 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer670) |
| Gene Information | human ... GRK2(156) |
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