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General description
Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag
Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.
Immunogen
Epitope: HHHHHH
a recombinant protein containing a His•Tag
Application
ELISA (0.1-0.4 g/ml)
Immunoblotting (1-4 g/ml)
Immunofluorescence (1-4 g/ml)
Immunoprecipitation (4 g/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note
Positive Control
Proteins containing a His•Tag
Other Notes
Antibody should be titrated for optimal results in individual systems.
Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347.
Gu, J., et al. 1994. BioTechniques17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol.68, 766.
Lu, T., et al. 1993. Anal. Biochem.213, 318.
Garner, J., et al. 1992. Cell69, 833.
Hochuli, E., et al. 1987. J. Chromatogr.411, 177.
Legal Information
Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 13/45/31/2, monoclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Calbiochem® |
| storage condition | do not freeze |
| dilution | (ELISA (0.1-0.4 µg/mL) Immunoblotting (1-4 µg/mL) Immunofluorescence (1-4 µg/mL) Immunoprecipitation (4 µg/mL)) |
| isotype | IgG1 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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