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General description
Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.
~43 kDa observed.
Immunogen
Epitope: pSer133.
KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB-A.
Application
Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor™ 488 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
The unconjugated antibody (Cat. No. 06-519) is shown to be suitable also for Electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry, and Western blotting applications.
Biochem/physiol Actions
Recognizes phosphorylated CREB-A (pSer133), htCREB (pSer133), and CREB-B (pSer119) spliced isoforms reported by (UniProt P16220). May also recognize phosphorylated CREM and ATF-1 that contain homologous sequence to that of the immungen peptide.
Physical form
Affinity purified
Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunofluorescence in rat brain tissue.
Immunofluorescence Analysis: A 1:50 dilution of this antibody detected phospho-CREB (Ser133) in rat brain tissue.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | ALEXA FLUOR™ 647 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, rat, mouse |
| species reactivity (predicted by homology) | Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology), chicken (based on 100% sequence homology), porcine (based on 100% sequence homology), guinea pig (based on 100% sequence homology) |
| technique(s) | immunofluorescence: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_604391 |
| UniProt accession no. | P16220 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer133) |
| Gene Information | human ... CREB1(1385) |
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