Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific,100UL,AB3832,Sigma

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订货号 0BV7074
品牌型号 Sigma AB3832
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

75KDa Moesin, and ~80kDa Ezrin & Radixin


Immunogen

Epitope: phospho-specific
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin


Application

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.

Immunohistochemistry (paraffin): 1:50

Optimal working dilutions must be determined by the end user.


Biochem/physiol Actions

Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.


Physical form

ImmunoAffinity Purified
Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.


Preparation Note

Maintain at -20°C for 12 months, do not aliquot.


Analysis Note

Control
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve


Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerabbit
Quality Segment100
antibody formaffinity isolated antibody
antibody product typeprimary antibodies
clonepolyclonal
purified byaffinity chromatography
species reactivitymonkey, human, mouse, rat
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry: suitable (paraffin),western blot: suitable
NCBI accession no.NM_004252.2
UniProt accession no.O14745
shipped indry ice
target post-translational modificationphosphorylation (pThr567)
Gene Informationhuman ... SLC9A3R1(9368)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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