Anti-Tie2 Mouse mAb (Ab33)，50UG，610205-50UGCN，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
Recognizes the ~140-45 kDa Tie2 protein.
This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.

Immunogen

Human
a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag sequence

Application

Frozen Sections (see comments)

Immunoblotting (0.2-1 µg/ml, chemiluminescence)

Immunoprecipitation (see comments)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

For frozen sections, tissues were treated with Triton^™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	mouse
Quality Segment	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	Ab33, monoclonal
form	liquid
contains	≤0.1% sodium azide as preservative
species reactivity	human, porcine, mouse, rat
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze,avoid repeated freeze/thaw cycles
dilution	(Frozen Sections Immunoblotting (0.2-1 µg/mL, chemiluminescence) Immunoprecipitation Paraffin Sections (not recommended))
isotype	IgG1
shipped in	wet ice
storage temp.	−20°C
target post-translational modification	unmodified
Gene Information	human ... TEK(7010)

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