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General description
Zinc finger protein ubi-d4 (UniProt: Q92785; also known as Apoptosis response zinc finger protein, BRG1-associated factor 45D, BAF45D, D4, zinc and double PHD fingers family 2, Protein requiem, DFP2) is encoded by the DPF2 (also known as BAF45D, REQ, UBID4) gene (Gene ID: 5977) in human. BAF45D is a member of the requiem/DPF protein family and is ubiquitously expressed. It may serve as a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. It is also believed to have a role in the development and maturation of lymphoid cells. It contains 3 zinc-finger domains (C2H2-type aa 209-232; PHD type I aa 270-330; and PHD type 2 aa 327-377). Two isoforms of BAF45D have been described that are generated via alternative splicing.
~44 kDa observed; 44.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated liner peptide that corresponding to 17 amino acids from the N-terminal region of human BRG1-associated factor 45D (DPF2).
Application
Anti-DPF2, Cat. No. ABE1327, is a highly specific rabbit polyclonal antibody that targets BRG1-associated factor 45D and has been tested in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: 2 µg/mL from a representative lot detected DPF2 in 10 µg of PC12 cell lysate.
Immunocytochemistry Analysis: A 1:100-500 dilution from a representative lot detected DPF2 in A431 and HepG2 cells.
Biochem/physiol Actions
This rabbit polyclonal antibody detects BRG1-associated factor 45D in human, mouse, and rat species. It targets an epitope within 17 amino acids from the N-terminal half. The immunogen sequence is conserved in both isoforms.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected DPF2 in 10 µg of NIH3T3 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, human, rat |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_006259.1 |
| UniProt accession no. | Q92785 |
| target post-translational modification | unmodified |
| Gene Information | human ... DPF2(5977) |
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmSFCA滤膜，孔径0.2um,适合瓶颈尺寸45mm，12/箱，291-4520，Nalgene，Thermofisher，赛默飞世尔¥ 2856.42
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