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Protein Phosphatase, Lambda, Recombinant, E. coli，20KU，539514-20KUCN，Sigma

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订货号： 0BV7195

品牌型号： Sigma 539514-20KUCN

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最小订货量： 1件

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产品介绍 Product Description

General description

Research Area: Cell Signaling
The protein phosphatase encoded by bacteriophage λ (λPP) is part of a family of Ser/Thr phosphatases (Ser/Thr PPases), which includes eukaryotic protein phosphatases 1 (PP1), 2A (PP2A), and 2B (calcineurin).
Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn²⁺-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units.

Application

Protein Phosphatase, Lambda, Recombinant, E. coli is suitable for the detection of phospho-KRAS.

Biochem/physiol Actions

Protein phosphatases play a crucial role in embryonic development, metabolic homeostasis, stress responses, cell cycle transitions, and numerous other vital biological processes.

Physical form

In 100 mM NaCl, 50 mM HEPES, 2 mM DTT, 0.1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.01% Brij^® 35 Detergent, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).
Further dilute in 50 mM Tris-HCl, pH 7.8, 5 mM DTT, 2 mM MnCl₂, and 100 µg/ml BSA.

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 30°C, pH 7.8. 20-200 Units will typically remove >95% of phosphates from serine/threonine residues in 0.1 nmol of protein in 30 min. 100-1000 Units will typically remove >95% of phosphates from tyrosine in 0.1 nmol of protein in 30 min.
Zhuo, S., et al. 1993. J. Biol. Chem.268, 17754.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Cohen, P.T.W. and Cohen, P. 1989. Biochem. J.260, 931.

Legal Information

Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

recombinant	expressed in E. coli
Quality Level	100
description	Activity (>= 400,000 units/mL)
assay	≥95% (SDS-PAGE)
form	liquid
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze,avoid repeated freeze/thaw cycles
foreign activity	Protease, none detected
shipped in	wet ice
storage temp.	−70°C

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