- 商品介绍
- 规格参数
- 包装参数
Immunogen
Synthetic peptide from the C terminus of human IP3 Receptor 2.
Application
Research Category
Signaling
Research Sub Category
Lipid Signaling
Detect IP3 Receptor 2 using this Anti-IP3 Receptor 2 Antibody validated for use in IC, IH & WB.
Western blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 2 protein in microsomal preparations. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel or 5% PAGE. For microsomal methods and blotting procedure similar antibodies can be found in: Perez, et al. J Biol. Chem. 272:23961
http://www.jbc.org/cgi/reprint/272/38/23961?ijkey=35bcf508a3025fc8bf8203ea3805122eec0caf1e )
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
IP3 Receptor 2.
Physical form
Rabbit serum. Liquid.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Western blot = adult mouse liver.
IHC = adult mouse hepatocytes (adult mouse cerebellum Purkinje cells will be negative).
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NM_002223.2 |
| UniProt accession no. | Q14571 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITPR2(3709) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
询价
-
¥ 2290.21
-
询价
-
¥ 9207.92
-
¥ 4039.86
-
询价
-
询价
-
询价
-
询价
-
¥ 4020.77
-
¥ 1783.21
-
¥ 3896.11
-
¥ 1351.48
-
¥ 2173.94
-
¥ 2911.61
-
¥ 9909.51
-
¥ 5990.26
-
询价
-
¥ 67162.32
微信小程序
7X24小时在线咨询