Protein A-Turbo - Proximity Biotinylation Enzyme，100UG，PAT001-100UG，Sigma

General description

Protein A Turbo is a recombinantly expressed Protein A-TurboID fusion protein with the capability to bind IgG through the Protein A region, and enzymatically biotinylate proximal proteins non-specifically with the TurboID region.

Application

• Proximity protein labeling
• Cell Biology
• Histology
• Fixed cell microscopy
• Tissue staining
• Immunofluorescence
• Liquid Chromatography
• Mass Spectrometry
• Immunoprecipitation

Biochem/physiol Actions

Protein A strongly binds multiple Immunoglobulin G subclasses (IgG1, IgG2, IgG4), but does not bind IgG3. This probe is not compatible with primary antibodies that are IgG3.

Physical form

Purified protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 150 mM imidazole with protease inhibitor cocktail.

Preparation Note

0.7 mg/mL
Store at -65°C to -96°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.