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General description
Affinity purified goat anti-mouse IgG, Biotin conjugated.
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Application
Detect Mouse IgG using this Goat anti-Mouse IgG Antibody, biotin-SP conjugate validated for use in ELISA & WB.
EIA: 1:50,000-1:100,000
Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000
Flow cytometry: 1:200-1:1,000
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Physical form
ImmunoAffinity Purified
PRESENTATION: Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.1% sodium azide.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label.
Preparation Note
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Level | 100 |
| conjugate | biotin conjugate |
| antibody form | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| species reactivity | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,western blot: suitable |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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