- 商品介绍
- 规格参数
- 包装参数
General description
33 kDa (Zhou, 1995)
CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells (Tedder, 1994). In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines (Zhou, 1995). The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface (Tedder, 1994). Antibodies reacting with CD20 cytoplasmic epitopes are designated CD20cy (Zhou, 1995). It is suggested that CD20 plays a direct role in regulating the transmembrane conductive Ca 2+ flux of B cells which indicates a possible function for CD20 as a regulator of proliferation and differentiation (Tedder, 1994).
Application
This Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1 is validated for use in FC for the detection of CD20.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Biochem/physiol Actions
Antibody recognizes Human CD20 antigen.
Physical form
Protein A purified
Purified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
Preparation Note
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Human Lymphocytes
Evaluated by flow cytometry using human lymphocytes.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | FITC conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | B-Ly1, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_690605.1 |
| UniProt accession no. | P11836 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MS4A1(931) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 2290.21
-
¥ 2013.69
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 4390.38
-
询价
-
¥ 10277.12
-
¥ 2617.87
-
¥ 1156.91
-
¥ 4011.97
-
¥ 1586.69
-
NALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/8 x 1/4 x 1/16，英寸，每箱数量50，50/箱，8000-0020，Thermofisher，赛默飞世尔
询价
-
¥ 3662.39
-
询价
微信小程序
7X24小时在线咨询