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General description
10 kDa calculated
Human alpha defensin-5 (HD5) is one of six peptides belonging to the alpha defensin family. Defensins are small peptides involved in immunological defense against viral and microbial infections through either direct interaction with pathogens or in combination with other immune system factors. There are three subfamilies of defensins (α, β, and θ). α defensins are only observed in mammals and are mainly β-sheet structures. Alpha defensin-5 (HD5) is primarily expressed in the Paneth cells found within the small intestine, and has been shown to possess activity against both Gram-positive and Gram-negative microbes. Studies have shown alpha defensin-5 (HD5) to be a strong antagonist towards human Papillomavirus infection. It may also play a role Crohn’s disease.
Immunogen
Recombinant protein corresponding to human alpha defensin-5 (HD5).
Application
Anti-Alpha Defensin-5 (HD5) Antibody, clone 8C8 detects level of Alpha Defensin-5 (HD5) & has been published & validated for use in IH & WB.
Western Blot Analysis: 0.5 ug/mL from a previous lot detected alpha defensin-5 (HD5) in human small intestine tissue lysate. The urea-treated method is recommended instead of SDS page (Shen, B. et al., 2005).
Physical form
Format: Purified
Analysis Note
Control
Normal human tranverse colon tissue
Evaluated by Immunohistochemistry in normal human tranverse colon tissue.
Immunohistochemistry Analysis: A 1:200 dilution of this antibody detected alpha defensin-5 (HD5) in normal human tranverse colon tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 8C8, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_066290 |
| UniProt accession no. | Q01523 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... DEFA5(1670) |
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