Anti-Gₛα-Subunit, C-Terminal (385-394) Rabbit pAb,50UL,371732-50ULCN,Sigma

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订货号 0BV7768
品牌型号 Sigma 371732-50ULCN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Anti-Gsα-Subunit, C-Terminal (385-394), rabbit polyclonal, recognizes both large and small forms of Gsα-subunit. It is validated for use in Western blotting.
Protein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa Gsα subunit protein.
Recognizes both large and small forms of Gsα-subunit. Does not cross-react with Giα-1-, Giα-2-, Giα-3-, or Goα-subunits.


Immunogen

a synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH


Application

Immunoblotting (1:1000)


Physical form

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.


Preparation Note

Following initial thaw, aliquot and freeze (-70°C).


Analysis Note

Positive Control
Gsα-Subunit, His•Tag, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)


Other Notes

Does not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Raymond, J.R., et al. 1993. Biochemistry32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
biological sourcerabbit
Quality Segment100
antibody formpurified antibody
antibody product typeprimary antibodies
clonepolyclonal
formliquid
does not contain preservative
species reactivity (predicted by homology)mammals
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze,avoid repeated freeze/thaw cycles
dilution (Immunoblotting (1:1000))
isotypeIgG
shipped inwet ice
storage temp.−70°C
target post-translational modificationunmodified
Gene Informationhuman ... GNAI1(2770)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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