- 商品介绍
- 规格参数
- 包装参数
General description
Sterol regulatory element-binding protein cleavage-activating protein (UniProt P97260; also known as SCAP, SREBP cleavage-activating protein) is encoded by the SCAP gene (Gene ID 100689048) in hamster species. SCAP mediates the activation of membrane-bound transcription factors SREBPs (sterol-regulatory element binding proteins) for transcribing genes encoding cholesterol biosynthetic enzymes. In sterol-deprived cells, SCAP escorts SREBPs from ER to Golgi, where active fragments of SREBPs are released from membrane by proteolysis. Sterol accumulation inuduces a negative regulatory mechanism, where SCAP becomes bound by Insigs and trapped in the ER, preventing further delivery of SREBPs to the Golgi for proteolytic release. SCAP spans the ER membrane eight times (a.a. 19-39, 280-300, 313-333, 345-365, 402-422, 424-444, 519-539, 709-729) with
4 lumenal and 5 cytoplasmic regions, having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-18 & 730-1276). The C-terminal domain of SCAP mediates association with SREBPs, while transmembrane helices 2–6 comprise the sterol-sensing domain that mediates sterol-induced binding of SCAP to Insigs. Mutations within the sterol-sensing region disrupt Insig binding and prevent sterol-mediated ER retention of SCAP-SREBP.
~150 kDa observed. Target band size appears larger than the calculated molecular weight of 139.5 kDa due to glycosylation. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: The fourth lumenal domain.
His-tagged recombinant fragment corresponding to the fourth lumenal domain of hamster SCAP.
Application
Anti-SCAP Antibody, clone 9D5 is an antibody against SCAP for use in Western Blotting, Immunocytochemistry.
Western Blotting Analysis: 5 µg/mL from a representative lot detected SCAP in 25 µg of whole cell lysate and 50 µg of membrane extract from CHO-K1 cells, but not from the SCAP-deficient SRD-13A CHO cells (Courtesy of Linda Donnelly, Department of Molecular Genetics, UT Southwestern Medical Center, Dallas, TX, USA).
Western Blotting Analysis: A representative lot detected exogenously expressed SCAP co-immunoprecipitated with the exogenously expressed Insig-1 and Insig-2 upon 25-hydroxycholesterol (25-HC) treatment of a SCAP-deficient CHO cell line SRD-13A that had been transfected to co-express SCAP with either myc-tagged Insig-1 or Insig-2 (Lee, P.C., and DeBose-Boyd, R.A. (2010). J. Lipid Res. 51(1):192-201).
Western Blotting Analysis: Representative lots detected similar level of SCAP in the membrane extracts from CHO cells with or without SREBP processing inhibitor 25-hydroxycholesterol (25-HC) treatment regardless whether the cells′ 25-HC-resistant status (Lee, P.C., and DeBose-Boyd, R.A. (2010). J. Lipid Res. 51(1):192-201; Lee, P.C., et al. (2007). J. Lipid Res. 48(9):1944-1954).
Western Blotting Analysis: A representative lot detected the endogenous SCAP immunoprecipitated from CHO cell lysate by a polyclonal SCAP antibody (Sakai, J., et al. (1997). J. Biol. Chem. 272(32):20213-20221).
Immunocytochemistry Analysis: A representative lot localized the overexpressed hamster SCAP at ER and nuclear envelope by fluorescent immunocytochemistry staining of 3% paraformaldehyde-fixed, 0.01% saponin-permeabilized CHO stable transfectants (Sakai, J., et al. (1997). J. Biol. Chem. 272(32):20213-20221).
Biochem/physiol Actions
Clone 9D5 detected the target band in lysate and membrane extract from CHO-K1 cells, but not from the SCAP-deficient SRD-13A CHO cell line.
Physical form
Format: Purified
Analysis Note
Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2bκ.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 9D5, monoclonal |
| species reactivity | hamster |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_001230965 |
| UniProt accession no. | P97260 |
| target post-translational modification | unmodified |
| Gene Information | hamster ... Scap(100689048) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
询价
-
¥ 2290.21
-
¥ 2814.26
-
¥ 3109.49
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 3442.25
-
¥ 8135.09
-
询价
-
¥ 238.17
-
瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmSFCA滤膜，孔径0.2um,适合瓶颈尺寸45mm，12/箱，291-4520，Nalgene，Thermofisher，赛默飞世尔¥ 2856.42
-
询价
-
¥ 14407.09
-
¥ 3335.66
-
¥ 2882.02
-
¥ 2666.57
微信小程序
7X24小时在线咨询