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General description
Agouti-related peptide-expressing (AgRP) neurons seem to be critical for the acute regulation of eating. The AgRP protein is a strong antagonis of MC3-R and MC4-R and is expressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
Immunogen
A 15 amino acid peptide near the C-terminus of mouse AGRP (Shutter et al. 1997; Ollmann et al. 1997).
Application
Detect Agouti Related Protein using this Anti-Agouti Related Protein Antibody validated for use in ELISA & WB.
Research Category
Metabolism
Research Sub Category
Glucose/Glycogen Metabolism
Western blot: 1-10 μg/mL using ECL.
ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide per well.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes AGRP. The immunogen shows no significant sequence homology with Agouti, tubby, tub, TULP1 or TULP2.
SPECIES REACTIVITIES: The immunogen sequence is 85% homologous with human and bovine. Reactivity with other species has not been confirmed.
Physical form
Liquid in phosphate buffered saline containing 0.1% BSA.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,western blot: suitable |
| NCBI accession no. | NM_001138.1,NM_007316.1 |
| UniProt accession no. | O00253 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... AGRP(181) |
| 长度(mm) | |
| 宽度(mm) | |
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