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General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle.
~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Immunogen
Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 phosphorylated at Tyr41.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho Histone H3 (Tyr41) in HeLa and NIH/3T3 cells.
DyLight® is a registered trademark of Thermo Fisher Scientific.
Dot Blot Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H3 (Tyr41) in an Absurance
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
This Anti-phospho Histone H3 (Tyr41) Antibody is validated for use in western blotting, ICC & dot blot for the detection of phospho Histone H3 (Tyr41).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in colcemid treated HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Tyr41) in 10 µg of colcemid treated HeLa acid extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse |
| technique(s) | dot blot: suitable,immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_003520 |
| UniProt accession no. | P68431 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pTyr41) |
| Gene Information | human ... HIST1H3F(8968) |
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