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General description
Derlin-1 (UniProt Q9BUN8; also known as Degradation in endoplasmic reticulum protein 1, Der1-like protein 1, DERtrin-1) is encoded by the DERL1 (also known as DER1) gene (ORF UNQ243/PRO276; Gene ID 79139) in human. Derlins are integral ER membrane proteins that form homo-oligomers and heteromultimeric complexes with membrane-bound machinery, including the E3 ubiquitin ligase Hrd1 and its binding partner SEL1L, involved in ER-associated degradation (ERAD). Before ERAD substrates can be processed by the cytoplasmic ubiquitin-proteasome system, they need to be translocated across the ER membrane through an AAA ATPase p97/VCP-driven process. Derlins are inactive members of the rhomboid family of intramembrane proteases that mediate the recruitment of AAA ATPase p97/VCP through interaction via their C-terminal SHP boxes. Derlin-1 is proposed to contain four transmembrane segments (a.a. 23-43, 60-80, 106-126, 155-175), three cytoplasmic domains (a.a. 23-43, 60-80, 106-126, 155-175), and two lumenol (a.a. 44-59 & 127-154) domains.
~24 kDa observed. 28.80 kDa (isoform 1) and 26.42 kDa (isoform 2) calculated.
Immunogen
Epitope: C-terminal cytoplasmic domain.
GST-tagged recombinant human derlin-1 C-terminal cytoplasmic fragment.
Application
Immunocytochemistry Analysis: An 1:50 dilution from a representative lot detected Derlin-1 in HUVECs, A431 and HeLa cells.
Research Category
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
This Anti-Derlin-1 Antibody, clone 8G11.2 is validated for use in Western Blotting, Immunocytochemistry for the detection of Derlin-1.
Biochem/physiol Actions
Expected to react with both spliced isoforms of human Derlin-1 reported by UniProt (Q9BUN8).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Derlin-1 in 10 µg of HEK293 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 8G11.2, monoclonal |
| species reactivity | human, mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_001128143 |
| UniProt accession no. | Q9BUN8 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... DERL1(79139) |
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