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General description
Protein S100-A8/9 (UniProt: P05109/ P06702; also known as MRP-8/MRP-14, Leukocyte L1 complex light chain/ Leukocyte L1 complex heavy chain, p8/p14, S100 calcium-binding protein A8/A9) are encoded by the S100A8/S100A9 (also known as MRP8/MRP14) genes (Gene ID: 6279/6280) in human. MRP-8 and MRP-14 are calcium- and zinc-binding protein that play a prominent role in the regulation of inflammatory processes and immune response. They are known to induce neutrophil chemotaxis and adhesion. They are predominantly found as calprotectin and perform a variety of intra- and extracellular functions. Their intracellular functions include facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes, and activation of the neutrophilic NADPH-oxidase. They are known to activate NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane. MRP-8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. Their extracellular functions include proinflammatory, antimicrobial, oxidant-scavenging and induction of apoptosis. MRP-14 is reported to induce neutrophil chemotaxis and adhesion and can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 pathways and can induce degranulation of neutrophils by a MAPK-dependent mechanism. MRP-8 and MRP-14 also acts as danger associated molecular pattern (DAMP) molecules and stimulate innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end (AGER) products. They also play an impotent role as oxidant scavengers and have a protective role in preventing exaggerated tissue damage by scavenging oxidants. (Ref.: Atallah, M., et al. (2012). PloS One. 7(2); e29333; Nakatani, Y., et al. (2005). Mediators Inflamm. 2005(5); 280-292; Ryckman, C., et al. (2003). J. Immunol. 170(6); 3233-3242).
Immunogen
Neutrophils
Application
Detect Migration Inhibitory Factor Related Proteins 8 & 14 using this Anti-Migration Inhibitory Factor Related Proteins 8 & 14 Antibody, clone AHN-17 validated for use in WB & IC.
Immunocytochemistry on ethanol fixed cells
Immunoblotting on un-reduced gels
Optimal dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Biochem/physiol Actions
Migration Inhibitory Factor Releasing Proteins -8 and -14
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL269
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | AHN-17, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_002964.3 |
| UniProt accession no. | P05109 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... S100A8(6279) |
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