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General description
Members of the SOX family of transcription factors are widely conserved and play important roles in early development and are often used as markers to assess the differentiation of specific cell lineages. SOX-15 (also known as SOX-20) is a nuclear, 25 kDa transcriptional repressor that belongs to group G of the SOX gene family. Sox-15 is widely expressed in fetal and adult tissues, with highest level found in fetal spinal cord, adult brain and adult testis. Recently, SOX-15 has been identified as having transcriptional control in mouse embryonic stem cells pluripotency along with SOX-2. SOX-15 is also expressed in myoblasts and regulates skeletal muscle regeneration and differentiation.
~ 36 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human SOX-15.
Application
Anti-SOX-15 Antibody, clone 1A2.1 is an antibody against SOX-15 for use in western blotting & ICC.
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected SOX-15 in a undifferentiated H9 human embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in a undifferentiated H9 human embryonic stem cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected SOX-15 in a undifferentiated H9 human embryonic stem cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 1A2.1, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_008873 |
| UniProt accession no. | O60248 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SOX12(6666) |
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