- 商品介绍
- 规格参数
- 包装参数
General description
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
~130 kDa
Immunogen
Epitope: C-terminus
Synthetic peptide corresponding to the c-terminal cytoplasmic domain of human β-1 integrin.
Application
Anti-Integrin beta1 Antibody, Cytosolic detects level of Integrin beta1 & has been published & validated for use in IP & WB.
Immunoprecipitation:
1 μL of antiserum per 25 μg total cell protein (in 40 μL). ) 0.5% triton X-100 lysis buffer with proteinase inhibitors is suggested.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
Biochem/physiol Actions
Reactive with human, mouse and rat integrin β-1, as confirmed by western blot. Not reactive with other integrins.
Physical form
Rabbit polyclonal serum containing no preservatives
Unpurified
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
U251 cell line
Western Blot Analysis:
1:500 to 1:1000 dilution of this antibody was used for detection of integrin beta1 in whole cell lysate under non-reducing. Size of beta 1 integrin under non-reduced conditions is approximately 110 kDa; often a 220 kDa dimer is observed as well; beta -1 under reduced conditions runs as a 130kDa band with higher molecular weight aggregates as well.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, rat, pig, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NM_002211.2 |
| UniProt accession no. | P05556 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGB1(3688) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 2814.26
-
¥ 1573.49
-
询价
-
¥ 4173.75
-
询价
-
询价
-
询价
-
¥ 3152.47
-
询价
-
询价
-
询价
-
¥ 1653.62
-
询价
-
询价
-
¥ 8982.64
-
¥ 2666.57
-
¥ 2617.87
-
¥ 1476.00
-
¥ 8137.12
-
¥ 5061.38
微信小程序
7X24小时在线咨询