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Anti-GNAT1 Antibody, clone 11A8.1，100UG，MABN1578，Sigma

销售价： ¥ 3501.72 / 件

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订货号： 0BV8231

品牌型号： Sigma MABN1578

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最小订货量： 1件

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产品介绍 Product Description

General description

Guanine nucleotide-binding protein G(t) subunit alpha-1 (UniProt P11488; also known as Transducin alpha-1 chain) is encoded by the GNAT1 (also known as GNATR, CSNB1G, CSNBAD3) gene (Gene ID 2779) in human. Heterotrimeric G proteins are activated by G protein-coupled receptors (GPCRs) and are composed of alpha (α), beta (β), and gamma (γ) subunits. There exists also monomeric Ras superfamily of small GTPases that are commonly referred to as small G-proteins. G-proteins mediate signal transduction initiated by GPCRs. Ligand binding activates GPCRs by inducing a conformational change. The activated GPCRs catalyze the GDP-to-GTP exchange of the Gα subunit, causing a dissociation of the Gβγ dimer from the now GTP-bound Gα subunit. The Gα subunit gradually hydrolyzes the bound GTP to GDP and eventually re-associates with Gβγ and re-starts the cycle. There exist at least 20 different Gα subunits, which are separated into four main groups, Gi, Gs, Gq, and G12/13. Eight different genes code for Gi alpha subunits, including GNAT1 and GNAT2 that code for Gt in the rod (rTα by GNAT1) and cone (cTα by GNAT2) cells of the retina, where they function as the alpha subunit of the trimeric G protein transducin. The α-subunit binds GTP and activates cyclic GMP phosphodiesterase (PDE), whereas the β and γ subunits mediate interaction with rhodopsin. Mutations in RHO, PDE6B, and GNAT1 genes are associated with autosomal dominant congenital stationary night blindness (CSNB), and patients with mutations in these genes exhibit the Riggs- or Schubert-Bornschein-type electronegative electroretinogram (ERG).
~40 kDa observed. 39.91 kDa calculated.

Immunogen

GST-tagged recombinant human GNAT1 internal fragment.

Application

Anti-GNAT1 Antibody, clone 11A8.1 is an antibody against GNAT1 for use in Western Blotting.

Biochem/physiol Actions

Clone 11A8.1 was raised against human Gt alpha-1 subunit (GNAT1) recombinant fragment. Cross-reactivity toward human Gt alpha-2 subunit (GNAT2) has not been determined.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GNAT1 in 10 µg of human retina tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

技术参数 Specifications

biological source	mouse
Quality Segment	100
conjugate	unconjugated
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	11A8.1, monoclonal
species reactivity	human
technique(s)	western blot: suitable
isotype	IgG2aκ
NCBI accession no.	NP_000163
UniProt accession no.	P11488
target post-translational modification	unmodified
Gene Information	human ... GNAT1(2779)

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