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- 规格参数
- 包装参数
General description
Podocalyxin (UniProt: Q9R0M4; also known as Podocalyxin-like protein 1, PC, PCLP-1) is encoded by the Podxl (also known as Pclp1) gene (Gene ID: 27205) in murine species. Podocalyxin is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 22-404), a transmembrane domain (aa 405-425), and a cytoplasmic domain (aa 426-503). Its expression is observed in podocytes, liver cells, hematopoietic progenitor cells, vascular endothelial cells, and a subset of neurons. It is present as a monomer but can oligomerize upon association with the membrane raft. Its O-glycan-rich domain in the extracellular region and the C-terminus PDZ-binding motif in the cytoplasmic tail harbor an apical sorting signal. Its cytoplasmic domain is reported to be essential for the apical membrane targeting and renal tubulogenesis. Its large highly anionic extracellular domain allows to maintain open filtration pathways between neighboring podocyte foot processes. The extracellular domain is also required for microvillus formation. In kidneys, it functions primarily as an anti-adhesive molecule in podocyte epithelial cells, regulating adhesion and cell morphology. In other tissues, it plays subtle roles in tissue remodeling and development. It also participates in a number of cellular functions through its association with the actin cytoskeleton, ezrin, and Na+/H+ exchanger regulatory factor 1 and 2 proteins. It is the most commonly used marker protein for detecting podocytes in urine under conditions such as IgA nephropathy, Henoch-Schönlein purpura, diabetic nephropathy, lupus nephritis, poststreptococcal glomerulonephritis, membranous nephropathy, and focal segmental glomerulosclerosis. Higher expression of podocalyxin is observed in many cancers, especially those that originated from the epithelium and is linked to poor prognosis, more aggressive tumor progression, and chemoresistance. (Ref.: Tran, NL., et al. (2021). Cancers (Basel). 1(12); 2863; Kruse, A., et al. (1999). Biol. Reprod. 61(6); 1393-1401).
Immunogen
Mouse lymph node stroma.
Application
Quality Control Testing
Evaluated by Western Blotting in bEnd.3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Podocalyxin in bEnd.3 cell lysate.
Tested applications
Immunofluorescence Analysis: A representative lot detected Podocalyxin in Immunofluorescence applications (Kruse, A., et al. (1999). Biol Reprod. 61(6):1393-401; Brulois, K., et al. (2020). Nat Commun. 11(1):3798).
Immunohistochemistry Applications: A representative lot detected Podocalyxin in Immunohistochemistry applications (Hallmann, R., et al. (1995). Dev Dyn. 202(4):325-32; Kruse, A., et al. (1999). Biol Reprod. 61(6):1393-401).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone MECA99 is a rat monoclonal antibody that detects Podocalyxin.
Physical form
Purified rat monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | MECA99, monoclonal |
| mol wt | calculated mol wt 53.39 kDa,observed mol wt ~140 kDa |
| purified by | using protein G |
| species reactivity | mouse |
| packaging | antibody small pack of 100 μg |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2cκ |
| epitope sequence | Unknown |
| Protein ID accession no. | NP_038751 |
| UniProt accession no. | Q9R0M4 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Podxl(27205) |
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