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Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)，20UG，OP43-20UGCN，Sigma

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订货号： 0BV8591

品牌型号： Sigma OP43-20UGCN

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最小订货量： 1件

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商品介绍
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产品介绍 Product Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

Immunogen

Epitope: Within amino acids 21-25 of human p53
Human
wild type, recombinant, human p53

Application

Frozen sections (1 g/ml; see application references)
Immunoblotting (0.1-1 g/ml; see application references)
Immunocytochemistry (1-2.5 g/ml; see application references)
Immunoprecipitation (1 g/ml or use Cat. No. OP43A; see application references)
Paraffin sections (1 g/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
SK-OV-3 cells or normal skin tissue
Positive Control
A431 cells or breast carcinoma tissue

Other Notes

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Vojtesek, B., et al. 1992. J. Immunol. Meth.151, 237.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.
Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with ³⁵S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	mouse
Quality Level	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	DO-1, monoclonal
form	liquid
contains	≤0.1% sodium azide as preservative
species reactivity	feline, human
should not react with	mouse, rat
manufacturer/tradename	Calbiochem^®
storage condition	do not freeze
dilution	(Frozen sections (1 µg/mL) Immunoblotting (0.1-1 µg/mL) Immunocytochemistry (1-2.5 µg/mL) Immunoprecipitation (1 µg/mL) Paraffin sections (1 µg/mL, pepsin, heat or pressure cooker pre-treatment required))
isotype	IgG2a
shipped in	wet ice
storage temp.	2-8°C
target post-translational modification	unmodified
Gene Information	human ... TP53(7157)

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