Tuner (DE3) Competent Cells - Novagen®,1ML,70623-4CN,Sigma

销售价: ¥ 2996.84 / 件
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订货号 0BV8599
品牌型号 Sigma 70623-4CN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Genotype: FompT hsdSB (rB mB) gal dcm lacY1(DE3)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.


Other Notes

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlTuner(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid


Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the “EMD Product”) requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Toxicity: Multiple Toxicity Values, refer to MSDS (O)


技术参数 Specifications
biological sourceEscherichia coli
Quality Segment100
manufacturer/tradenameNovagen®
storage conditionOK to freeze,avoid repeated freeze/thaw cycles
growth modeadherent or suspension
morphologyrod shaped
technique(s)microbiological culture: suitable
cell transformationtransformation efficiency: >2×106 cfu/μg
shipped indry ice,wet ice
storage temp.−70°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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