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General description
Bromodomain PHD finger transcription factor (UniProt: E9Q6A7; also known as Nucleosome-remodeling factor subunit BPTF; Fetal Alz-50 clone 1 protein, Fetal Alzheimer antigen) is encoded by the Bptf (also known as Fac1, Falz) gene (Gene ID: 207165) in murine species. BPTF, SNF2L and pRBAP46/48 are the three subunits of the nucleosome-remodelling factor NURF, an ISWI chromatin-remodeling complex that catalyzes ATP-dependent nucleosome sliding. BPTF mediates NURF target site specificity via interactions with transcription factors, histone variants and histone modifications on transcriptionally active genes. BPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. BPTF is necessary for the survival of c-MYC-overexpressing cells and for c-MYC-driven tumorigenesis in the mouse pancreas. BPTF knockdown leads to decreased c-MYC chromatin accessibility and significantly delays tumour development in pre-neoplastic pancreatic acinar cells. Consistently, BPTF expression in human tumours positively correlates with activation of c-MYC gene signatures.
Target molecular weight ~250 kDa observed. 321.6 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST-tagged recombinant N-terminal fragment of mouse BPTF.
Application
Anti-BPTF, Cat. No. ABE24, is a rabbit polyclonal antibody that detects BPTF and is tested for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, and Peptide Inhibition Assay.
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected BPTF in NIH/3T3 cell lysate.
Tested applications
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected BPTF in MEF-1 and mESC cell lysates.
Immunoprecipitation: A representative lot immunoprecipitated BPTF in Immunoprecipitation application (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected BPTF in A431 cells.
Peptide Inhibition Assay: Target band detection in lysate from NIH/3T3 cells was prevented by pre-blocking of a representative lot with recombinant fragment of mouse BPTF.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Research Category
Epigenetics & Nuclear Function
Biochem/physiol Actions
This rabbit polyclonal antibody detects BPTF. It targets an epitope within 103 amino acids from the N-terminal half.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_789820 |
| UniProt accession no. | A2A654,E9Q6A7 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... BPTF(2186) mouse ... Bptf(207165) |
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