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General description
Fibulin-1 (UniProt P23142; also known as FIBL-1) is encoded by the FBLN1 (also known as FIBL1, FBLN, PP213) gene (Gene ID 2192) in human. Fibulin-1 is an extracellular matrix protein that interacts with fibronectin and inhibits cell migration and invasion in a fibronectin-dependent manner. Research shows that the EGF-like domains 5 and 6 of fibulin-1 compete against syndecan-4 for binding the C-terminal heparin-binding domain (HepII) of fibronectin, resulting in a suppression of fibronectin-regulated cell adhesion and motility. Fibulin-1 also interacts with fibrinogen and regulates platelet adhesion to influence hemoglutination. In addition, fibulin-1 interacts with elastin and plays a role in the assembly of elastic fibers by mediating association between versican and fibrillin. Other fibulin-1 interactors include calumenin, extracellular matrix protein 1, angiogenin, and hemicentin. There exist four major fibulin-1 isoforms (A, B, C, and D) generated by alternative splicing, with isoforms C and D being the predominant forms. In addition, a smaller isoform of fibulin-1D, fibulin-1D′, is reported to localize in the cytosol and interacts with the intracellular domain of integrin β1. Fibulin-1 is initially produced with a signal peptide (a.a. 1-29), the removal of which yields the mature protein with three N-terminal Anaphylatoxin-like domains (a.a. 36-76, 77-111, 112-144), followed by eight EGF-like domains (a.a. 176-578 of isoform D), with EGF-like domains 5 & 6 mediating fibulin-1 self-association and fibronectin-binding. The C-terminal 137-amino acid in isoform D is truncated in isoform A, and replaced by a shorter sequence of 35 amino acids in isoform B and a sequence of 117 amino acids in isoform C.
~77 kDa observed. 77.2 kDa (isoform D), 61.6 kDa (isoform A), 65.4 kDa (isoform B), and 74.4 kDa (Isoform C) calculated.
Immunogen
Epitope: EGF-like domains 4-6.
GST-tagged recombinant protein corresponding to EGF-like domains 4-6 of human Fibulin-1.
Application
Detect Fibulin-1 using this Anti-Fibulin-1 Antibody, clone 3D12.1 validated for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Fibulin-1 in human placenta and human breast tissue.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Biochem/physiol Actions
Expected to react with all four spliced isoforms of human fibulin-1 (UniProt P23142-1 to P23142-4).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: 4.0 µg/mL of this antibody detected Fibulin-1 in 10 µg of SH-SY5Y cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3D12.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001987 |
| UniProt accession no. | P23142 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FBLN1(2192) |
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