- 商品介绍
- 规格参数
- 包装参数
General description
Ninein is a coiled-coil, centrosomal protein that is a necessary component in microtubule minus-end anchorage and positioning, and may have a role as a maturation factor for centrosomes. It may also be involved in microtubule nucleation. More recent studies have identified ninein as a crucial component in the formation of blood vessels. Expression of ninein is ubiquitous, but significantly higher levels have been observed in skeletal muscle and heart tissue. There have been 8 isoforms characterized due to alternative splicing.
~ 250 kDa observed MW. An unidentified nonspecific band appears at ~ 50 kDa
Immunogen
Recombinant protein corresponding to human Ninein.
Application
Anti-Ninein Antibody, clone 79-160-7 is an antibody against Ninein for use in WB & IP.
Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect Ninein in HeLa and A431 cells.
Immunoprecipitation Analysis: The antibody has been shown to immunoprecipitate Ninein in L929 cells. (Delgehyr, 2005).
Physical form
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.
Analysis Note
Evaluated by Western Blot in Hek293 cell lysate.
Western Blot Analysis: 1 µg/mL of the antibody detected Ninein in 10 µg of Hek293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 79-160-7, monoclonal |
| species reactivity | human |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_891989 |
| UniProt accession no. | Q8N4C6 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NIN(51199) |
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¥ 2019.57
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¥ 1451.54
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