- 商品介绍
- 规格参数
- 包装参数
General description
Mucins are a family of highly glycosylated proteins that are present on the surface of, and are secreted by, many glandular epithelial cells. Mucins provide a barrier between the luminal membranes of cells in the epithelium and their environment. For instance, in the digestive tract, the mucin barrier protects the epithelial cells from the action of proteolytic enzymes. These high molecular weight glycoproteins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. Salivary Mucin 5B proteins are some of the largest polypeptides, with molecular weights ranging from 14 - 40 x 103 kDa.
Immunogen
Un-glycosylated, serine- and threonine-free synthetic peptide from the central region of human MUC5B.
Application
Research Category
Apoptosis & Cancer
Research Sub Category
Tumor Markers
This Anti-Mucin 5B Antibody, clone 15.5B is validated for use in ELISA, WB, IH for the detection of Mucin 5B.
Western blot: Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins.
Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).
ELISA
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 15.5B, monoclonal |
| species reactivity | human, monkey |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2b |
| NCBI accession no. | NM_002458.1 |
| UniProt accession no. | Q9HC84 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MUC5B(727897) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 2622.09
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmPES滤膜，孔径0.45um,适合瓶颈尺寸33mm，12/箱，295-3345，Nalgene，Thermofisher，赛默飞世尔¥ 1938.54
-
¥ 1568.60
-
¥ 2842.54
-
¥ 5014.59
-
¥ 1586.69
-
¥ 3476.87
-
¥ 5426.00
-
询价
-
询价
-
¥ 37675.92
-
¥ 17652.29
微信小程序
7X24小时在线咨询