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General description
RAD50 (previously known as DNA repair protein RAD50) is homologous to Saccharomyces cerevisiae Rad50 and is a member of the structural maintenance of chromosome (SMC) family and is involved in cell growth and viability. RAD50 is known to encode 2 distinct proteins. The RAD50, XRS2/NBS1 complex is required for activation of ATM which will phosphorylate TERF1, thereby promoting telomerase. Along with MRE11 and NBN, RAD50 also forms the MRE11–RAD50–NBS1 (MRN) complex, which is one of the first factors to be localized to the DNA lesion, where it might initially have a structural role by tethering together, and therefore stabilizing, broken chromosomes. MRN also controls ATR, which is required for SMC1A phosphorylation, resulting in chromatid adhesion and DNA damage response. RAD50 is expressed in all tissue but predominantly in the testis and fibroblasts.
~154 kDa
Immunogen
Synthetic peptide corresponding to RAD50.
Application
Immunocytochemistry:
A 1:500 dilution of this antibody was used to detect RAD50 on HeLa and A431 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-RAD50 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB to detect RAD50 also known as RAD50 (S. cerevisiae) homolog.
Biochem/physiol Actions
This antibody recognizes RAD50.
Physical form
Affinity purified
Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.
Analysis Note
Control
Human spleen lysate.
Western Blot Analysis:
A 1:500 dilution of this antibody was used to detect RAD50 on human spleen lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat, mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_005723 |
| UniProt accession no. | Q92878 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RAD50(10111) mouse ... Rad50(19360) |
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