- 商品介绍
- 规格参数
- 包装参数
General description
Short-wave-sensitive opsin 1 (UniProt: P51491; also known as S opsin, Blue cone photoreceptor pigment, Blue-sensitive opsin, BOP, Short wavelength-sensitive cone opsin) is encoded by the Opn1sw (also known as Bcp) gene (Gene ID: 12057) in murine species. Opsins are G-protein coupled protein with photoreceptor activity. Opsins contain seven transmembrane alpha-helical domains connected by three extra-cellular and three cytoplasmic loops. They are light-sensitive proteins found in photoreceptor cells of the retina. Based on sequence homology, the opsins are classified into six subfamilies: the vertebrate opsin/encephalopsin subfamily, the Go opsin subfamily, the Gs opsin subfamily, the invertebrate Gq opsin subfamily, the photoisomerase subfamily, and the neuropsin subfamily. They share less than 20 percent identity between subfamilies. Opsins provide the necessary environment for the absorption of light at a particular wavelength. Therefore, by providing a different opsin to the retinal, organisms can sense light of different wavelengths. S-opsin is a multi-pass membrane protein that is expressed in cone photoreceptor cells. They are light-absorbing molecules that mediate vision and contain an apoprotein, opsin, which is covalently linked to cis-retinal. It can increase spectral sensitivity in dim light.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of murine short-wave-sensitive opsin 1.
Application
Quality Control Testing
Evaluated by Western Blotting in NRL knockout mouse retina lysate
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected S-Opsin in NRL knockout mouse retina tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected S-Opsin in mouse retina tissue sections.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected S-Opsin in Mouse Eye tissue lysate.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-S-Opsin , Cat. No. ABN1660-I, is a rabbit polyclonal antibody that detects S-Opsin (Short-wave-sensitive opsin 1) and is tested for use in Immunohistochemistry (Paraffin), Western Blotting, and Western Blotting using KO lysates.
Biochem/physiol Actions
This rabbit polyclonal antibody specifically detects S-Opsin (short-wave-sensitive opsin 1). It targets an epitope within 17 amino acids from the C-terminal half.
Physical form
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Preparation Note
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | Unknown, polyclonal,polyclonal |
| mol wt | calculated mol wt 38.92 kDa,observed mol wt ~37 kDa |
| purified by | affinity chromatography |
| species reactivity | mouse |
| species reactivity (predicted by homology) | rat |
| packaging | antibody small pack of 100 μg |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_031564 |
| UniProt accession no. | P51491 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | rabbit ... Pun1sw(12057) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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