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General description
ICA 512, also known as Receptor-type tyrosine-protein phosphatase-like N (R-PTP-N), or Islet cell antigen 512 (ICA 512) or Islet cell autoantigen 3, or PTPIA-2, or IA-2, and encoded by the gene PTPRN/ICA3/ICA512, is a required protein for endocrine and neuroendocrine secretory processes. ICA 512 may be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking and regulated exocytosis, or ICA 512 may have a more general role in neuroendocrine functions. ICA 512 interacts with phosphorylated syntrophin beta 2/Distrophin, protecting it from protein cleavage by calpain I. Dephosphorylation of Distrophin upon insulin stimulation disrupts the interaction and results in its cleavage. ICA 512 is a membrane protein and found within neuroendocrine secretory granules and endocrine tissues. ICA 512 is expressed in brain, pituitary and gut tissues including the pancreas. ICA 512 is also now used as a diabetes-specific islet autoantigen biomarker in type diabetes patients.
~65 kDa observed. The full length protein is cleaved to yield a 65 kDa mature protein form that contains a lumenal domain, and an intracellular cytoplasmic domain characterized by a juxtamembrane region (Piquer, S., et al. (2006). J Autoimmun. 26(3):215-222.).
Immunogen
Epitope: This antibody recognizes residues 626-630 found in juxtamembrane region of human and mouse IA-2, but not in IA-2b.
His-tagged recombinant protein corresponding to the residues 626-630 found in juxtamembrane region of human and mouse IA-2, but not IA-2b of human IA-2.
Application
Research Sub Category
Signaling Neuroscience
Anti-IA-2 antibody, clone 76F is an antibody against IA-2 for use in western blotting, IHC & IP.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected IA-2 in rat pancreas tissues and BTC3 cells (Piquer, S., et al. (2006). J Autoimmun. 26(3):215-222.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated IA-2 from recombinant rat, mouse, and human IA-2 (Piquer, S., et al. (2006). J Autoimmun. 26(3):215-222.).
Research Category
Signaling
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human pancreas tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected IA-2 in 10 µg of human pancreas tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 76F, monoclonal |
| species reactivity | mouse, human, rat |
| technique(s) | immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_001186692 |
| UniProt accession no. | Q16849 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PTPRN(5798) |
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