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General description
Eukaryotic translation initiation factor 4E (UniProt: P06730; also known as eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein) is encoded by the EIF4E (also known as EIF4EL1, EIF4F) gene (Gene ID: 1977) in human. elF-4E is a cytoplasmic factor that recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex that binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex. Three isoforms of elf-4E have been described that are generated by alternative splicing. Elf-4E contains four 7-methylguanosine-containing mRNA cap binding regions and three EIF4EBP1/2/3 binding regions. Hypophosphorylated EIF4EBP1, EIF4EBP2, and EIF4EBP3 are shown to compete with EIF4G1/EIF4G3 to interact with elF-4E. Phosphorylation of ElF4EBP1 by insulin-stimulated MAP kinase (MAPK1 and MAPK3) cause dissociation of the complex and allows EIF4G1/EIF4G3 to bind and result in initiation of translation. Mutations in ElF4E gene have been linked to autism with impairments in social interaction and communication and developmental abnormalities.
~31 kDa observed; 25.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: unknown
KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Eukaryotic translation initiation factor 4E.
Application
Anti-eIF-4E, Cat. No. ABE2695, is a highly specific rabbit polyclonal antibody that targets Eukaryotic translation initiation factor 4E and has been tested in Immunocytochemistry and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 0.9 µg/mL from a representative lot detected eIF-4E in 10 µg of PC12 cell lysate.
Immunocytochemistry Analysis: A 1:100-500 dilution from a representative lot detected eIF-4E in HeLa, A431, HUVEC, and NIH/3T3 cell lines.
Biochem/physiol Actions
This rabbit polyclonal antibody detects Eukaryotic translation initiation factor 4E in Human, Mouse, and Rat cells. it targets an epitope with in 14 amino acids from the N-terminal region.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected eIF-4E in 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | monkey (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_001124150 |
| UniProt accession no. | P06730 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... EIF4E(1977) |
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