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General description
Sentrin-specific protease 1 (EC 3.4.22.68; UniProt Q9P0U3; also known as Sentrin/SUMO-specific protease SENP1) is encoded by the SENP1 gene (Gene ID 29843) in human. SUMOylation, protein posttranslation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. As is the case with ubiquitination, SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while a family of SUMO-specific proteases (SENPs) are responsible for the deSUMOylation. Seven SENPs are identified in human (SENP1-3 and SENP5-8). SENP1 processes full-length SUMO1, SUMO2 and SUMO3 to their mature forms and mediates their removal (deconjugation) from substrate proteins, including BHLHE40/DEC1, CCAR2, CLOCK, DBC1, HADC1, HIPK2, MTA1, p53, and SIRT1. SENP1 is reported to be overexpressed and induced by IL-6 in multiple myeloma (MM) cells, and SENP1-knockdown triggers apoptosis and reduces viability, proliferation and colony forming ability of MM cells.
~73 kDa observed. 73.48 kDa (isoform 1), 73.35 kDa (isoform 2) calculated.
Immunogen
Epitope: Internal (C-terminal half).
GST-tagged recombinant fragment from the C-terminal half of human SENP1.
Application
Anti-SENP1 Antibody, clone 8B6.1 is an antibody against SENP1 for use in Western Blotting.
Research Category
Signaling
Research Sub Category
General Post-translation Modification
Biochem/physiol Actions
Expected to react with both spliced isoforms of human SENP1 reported by UniProt (Q9P0U3).
Physical form
Mouse monoclonal IgG2bλ hybridoma culture supernatant with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected SENP1 in 10 µg of HepG2 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | 8B6.1, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG2bλ |
| NCBI accession no. | NP_001254523 |
| UniProt accession no. | Q9P0U3 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SENP1(29843) |
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