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General description
220 kDa Calculated
SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Immunogen
Epitope: MC-480
F9 tetracarcinoma stem cells (X-irradiated).
Application
Anti-SSEA-1 Antibody, clone MC-480, PE conjugate is an antibody against SSEA-1 for use in IH, IP, IF, FC.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Biochem/physiol Actions
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Physical form
Purified mouse monoclonal IgM conjugated to PE fluorochrome in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Preparation Note
Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
Analysis Note
Control
Mouse embryonic Stem Cells
Evaluated by Flow Cytometry with mouse embryonic stem cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | PE |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | MC-480, monoclonal |
| species reactivity | rat, mouse, human |
| technique(s) | flow cytometry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgM |
| NCBI accession no. | NM_002033.2 |
| UniProt accession no. | P22083 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FUT4(2526) |
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