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- 规格参数
- 包装参数
General description
GAL4 is a transcriptional activator which controls the expression of genes involved in the catabolism of galactose. In addition to a DNA-binding domain, Gal4 contains two acidic regions: activating region I (aa148-238) and activating region II (aa768-881), which can both activate transcription when independently joined to the DNA-binding domain. The activation domains of Gal4 have been shown to interact with Gal80, TBP, SUG1, and ADA2, among other general transcription factors.
~28 kDa, ~38 kDa, ~77 kDa observed
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of yeast GAL4 (activation domain).
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-GAL4 (activation domain) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect GAL4 (activation domain) also known as Regulatory protein GAL4.
Biochem/physiol Actions
This antibody recognizes the C-terminus of GAL4 (activation domain).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Gal4 positive and Gal4 negative yeast
Evaluated by Western Blot in Gal4 positive and Gal4 negative yeast cell lysate.
Western Blot Analysis: A 1:160,000 dilution of this antibody detected Gal4 in 5 mg of Gal4 positive (+ recombinant Gal4) yeast cell lysate. This antibody also detected small mounts of endogenous Gal4 in Gal4 negative (- recombinant Gal4) yeast cell lysate.
Other Notes
Replaces: 06-283
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | yeast |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_015076 |
| UniProt accession no. | P04386 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | Saccharomyces cerevisiae ... Gal4(855828) |
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